FAQs

Do you have literature references about the use of fluorescein for the ORAC assay?
We are the first group who reported the use of fluorescein for  both water-soluble and fat-soluble antioxidants. Please feel free to request a copy of our peer-reviewed papers published in the Journal of Agricultural and Food Chemistry1,2,3.

1 Ou, B.; Hampsch-Woodill, M.; Prior, R. L. Development and validation of an improved oxygen radical oxygen assay using fluorescein as the fluorescent Probe. J. Agric.Food Chem. 2001, 49, 4619-4626.

2 Huang, D., Ou, B., Hampsch-Woodill, M. Development and validation of oxygen radical absorbance capacity assay for lipophilic antioxidants using randomly methylated beta-cyclodextrin as the solubility enhancer J. Agric.Food Chem. 2002, in press.

3 Huang, D., Ou, B., Hampsch-Woodill, M., Prior, RL Measurement of hydroxyl radical prevention capacity. J. Agric.Food Chem. 2002, in press


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Top of Page

What does "ORAC" stand for?
Who are the original ORAC developers?
What is the principle of the ORAC assay?
What does the ORAC assay actually measure?
What are the drawbacks of the original ORAC method?
What improvement on ORAC was made by Brunswick Laboratories?
Do you have literature references about the use of fluorescein for the ORAC assay?
How many harmful reactive oxygen species exist in the body?
Does the improved ORAC assay measure the total antioxidant activity against all the radicals?
What samples can be tested for ORAC?
What are the Applications of ORAC:
How long does the test take to perform?
What is the standard used in the ORAC assay?
What is the unit of the ORAC result?
Can I use the ORAC result as a quality control for my product development?
Can I use the published ORAC data for comparison purpose?
Why the ORAC assay is advantageous over many other methods?
What are the potential interferences/ pitfalls of this assay?
What instrument is used for the ORAC assay?
Who are the competitors for the ORAC testing service?
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