FAQs

Why the ORAC assay is advantageous over many other methods?
The mechanism of ORAC is based upon the sound chemical principle and the uniqueness of ORAC lies in the AUC quantitation technique. Indeed, many other methods have been developed for antioxidant activity, such as TEAC (Trolox Equivalent Antioxidant Capacity)1, TOSC (Total Oxyradical Scavenging Capacity)2, FRAP (Ferric Reducing Antioxidant Power)3, and DPPH method4. However, the fatal drawback of these methods is either no oxygen radical involved or lack of complete quantitation technique.  Therefore, only ORAC provides antioxidant activity mechanistically and physiologically.

1 Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 1999, 26, 1231-1237.

Winston, G. W.; Regoli, F.; Dugas, A. J.; Fong, J. H.; Blanchard, K. A. A rapid gas chromatographic assay for determining oxyradical scavenging capacity of antioxidants and biological fluids. Free Radic. Biol. Med. 1998, 24, 480-493.

Benzie, I. F. F.; Strain, J. J. The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay. Anal. Biochem. 1996, 239, 70-76.

Hirano R., Sasamoto W., Matsumoto A. Antioxidant ability of various flavonoids against DPPH radicals and LDL oxidation. J NUTR SCI VITAMINOL. 2001, 47, 357-362.


© Copyright 2003-2006 by Brunswick Laboratories

Top of Page

What does "ORAC" stand for?
Who are the original ORAC developers?
What is the principle of the ORAC assay?
What does the ORAC assay actually measure?
What are the drawbacks of the original ORAC method?
What improvement on ORAC was made by Brunswick Laboratories?
Do you have literature references about the use of fluorescein for the ORAC assay?
How many harmful reactive oxygen species exist in the body?
Does the improved ORAC assay measure the total antioxidant activity against all the radicals?
What samples can be tested for ORAC?
What are the Applications of ORAC:
How long does the test take to perform?
What is the standard used in the ORAC assay?
What is the unit of the ORAC result?
Can I use the ORAC result as a quality control for my product development?
Can I use the published ORAC data for comparison purpose?
Why the ORAC assay is advantageous over many other methods?
What are the potential interferences/ pitfalls of this assay?
What instrument is used for the ORAC assay?
Who are the competitors for the ORAC testing service?
  SEARCH