The Chemistry behind Antioxidant Capacity Assays

This review summarizes the multifaceted aspects of antioxidant analyses and the basic kinetic models of inhibited autoxidation.The chemical principles of the most popular assays, including inhibition of induced low-density lipoprotein autoxidation, ORAC, TRAP, crocin bleaching assays, FCR, TEAC, FRAP, "total antioxidant potential" assay using a Cu(II) complex as an oxidant, and DPPH are discussed. On the basis of this analysis, it is suggested that the total phenols assay by FCR be used to quantify an antioxidant's reducing capacity and the ORAC assay to quantify peroxyl radical scavenging capacity. To comprehensively study different aspects of antioxidants, validated and specific assays are needed, in addition to these two commonly accepted assays.

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Lipophilic and Hydrophilic Antioxidant Capacities of Common Foods in the United States
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Anthocyanin and Proanthocyanidin Content in Selected White and Red Wines
Proanthocyanidins are oligomers or polymers of polyhydroxy flavan-3-ol units (49). The flavan-3-ol class of phenols is responsible for both bitterness and astringency in wine... [ Download/View File ]
Chemical Composition of Caneberry (Rubus spp.) Seeds and Oils and Their Antioxidant Potential

This investigation has described compositional characteristics for seeds of five commonly grown caneberry species: red raspberry, black raspberry, boysenberry, Marion blackberry, and evergreen blackberry. Antioxidant capacities were detected both for whole seeds and for cold-pressed oils. This could lead to the development of a value-added use for Caneberries seeds, and thus expand the market for caneberries and the profit margins for growers.

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Assays for Hydrophilic and Lipophilic Antioxidant Capacity of Plasma
There is increasing interest in the use and measurement of antioxidant capacity in the food and pharmaceutical industries and in clinical studies. This interest is derived from the overwhelming evidence of the importance of... [ Download/View File ]
Antioxidant Activity and Phenolic Content of Oregon Caneberries
High intakes of fruits and vegetables have been associated with lower incidences of [ Download/View File ]
When east meets west
Ancient traditional Chinese medicine (TCM) has effectively relied on the theory of yin-yang... [ Download/View File ]
High-Throughput Assay of Oxygen Radical Absorbance
The oxygen radical absorbance capacity (ORAC) assay has been widely accepted as a... [ Download/View File ]
Analysis of Antioxidant Activities of Common Vegetables
A total of 927 freeze-dried vegetable samples, including 111 white cabbages, 59 carrots... [ Download/View File ]
Development and Validation of Lipophilic ORAC
Were cently reported the improved oxygen radical absorbance capacity (ORAC) assay using... [ Download/View File ]
Novel Fluorometric Assay for Hydroxyl Radical Prevention
A novel fluorometric method has been developed to evaluate hydroxyl radical prevention capacity... [ Download/View File ]
Improved Oxygen Absorbance Capacity Assay
An improved method of oxygen radical absorbance capacity (ORAC) assay has been developed and... [ Download/View File ]

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