Oxygen Radical Absorbance Capacity (ORAC) tests are among the most acknowledged methods to quantify antioxidants in a material. ORAC tests measure antioxidant scavenging activity against oxygen radicals that are known to be involved in the pathogenesis of aging and common diseases. ORAC 6.0™ consists of six types of ORAC assays that evaluate the antioxidant capacity of a material against six primary reactive oxygen or nitrogen species (ROSs or RNSs, commonly called “oxygen radicals” or ROSs) found in humans: peroxyl radical, hydroxyl radical, superoxide anion, singlet oxygen, peroxynitrite, and hypochlorite.
ORAC 6.0 assays are based on evaluating the capacity of a test material to protect a probe (e.g., a chromagen) from its damage by oxygen radicals. In all ORAC assays, an ROS inducer is introduced to the assay system. The ROS inducer triggers the release of a specific ROS, which would degrade the probe and cause its emission intensity or wavelength to change. When an antioxidant material presents in the environment, the antioxidant absorbs the ROS and preserves the probe from degradation. The degree of probe preservation indicates the antioxidant capacity of the material.
In all ORAC assays, the results are expressed as micromole Trolox Equivalency per gram (or milliliter) of a test material.
ORAC 6.0 includes the following individual ORAC tests:
- ORAC Assay against Peroxyl Radical
- ORAC Assay against Hydroxyl Radical (aka HORAC)
- ORAC Assay against Peroxynitrite (aka NORAC)
- ORAC Assay against Superoxide (aka SORAC)
- ORAC Assay against Singlet Oxygen (aka SOAC)
- ORAC Assay against Hypochlorite (aka CORAC)
Further Analysis: Antioxidant Bioavailability Assays
- Cellular Nrf2 Assay
- Cellular Antioxidant Assay (CAA)
- Caco-2 Permeability Assay: bioavailability study via in vitro human intestinal membrane
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