Wrinkles are a product of aging, smoking, sun exposure, pollution, and other factors. Damage and loss of collagen (which provides skin firmness), elastin (which supplies skin elasticity and rebound) and glycosaminoglycans (which keep the skin hydrated) result in the appearance of roughness, uneven tone, brown patches, thin skin and deep wrinkles. Prevention methods include sun protection, oral and topical use of antioxidants, cessation of smoking, and improved diet. A product’s wrinkle prevention potential can be analyzed, in part, by its ability to sustain the level of collagen and elastin in skin. Brunswick Labs offers below analyses to address these two objectives:
The collagen content of skin is the net balance between collagen synthesis and collagen breakdown. Age reduces collagen synthesis in human tissues including the skin. Environmental stress such as smoking, UV exposure, pollution and inflammation stimulate the production of collagen-degrading enzymes that causes collagen breakdown. Collagenase, one of the key collagen-degrading enzymes also called matrix metalloproteases (MMPs), is considered to be one of the central causes of skin aging. Direct inhibition of collagenase by plant compounds has been discovered an effective approach to mitigate collagen breakdown in the skin, improving skin health. Collagenase inhibition investigation assesses the impact of a test material on collagenase inhibition. Maximum degree of inhibition and concentration used will be reported. If available, EC50 (effective concentration at 50% of maximal inhibition) will also be reported.
Wrinkle formation in the skin is accompanied by decrease in skin elasticity and the curling of elastic fibers in the dermis. Elastase inhibitors suppress elastase activity and prevent the damage of dermal elastic fibers, thus helping mitigate wrinkle formation. In this investigation, impact of a test material on elastase inhibition is assessed. Maximum degree of inhibition and concentration used will be reported. If available, EC50 (effective concentration at 50% of maximal inhibition) will also be reported.