Frequently Asked Questions
Q: We know about ORAC. What is CAA?
A: Cellular antioxidant assay (CAA) is a cell-based method that uses human cells to measure biological outcomes. Cell culture assays are primary tools in biomedical research. They provide meaningful pre-clinical data on how substances react with living cells. The CAA test measures the ability of substances to neutralize radicals in living cells.
Q: Why is the ORAC/CAA combo meaningful?
A: For over a decade, ORAC has been a unique tool for quantifying antioxidant activity in sample materials. It has helped us better understand and identify antioxidant sources in natural products. However, as an in vitro chemical test, it does not describe how antioxidant substances perform in a living biological environment. By combining ORAC with CAA, we can investigate the correlation between in vitro ORAC results and antioxidant results in living cells.
Q: What correlations have you seen between ORAC and CAA so far?
A: We have seen a general positive correlation that demonstrates the cellular efficacy of antioxidant substances. In other words, a variety of antioxidant sources that have high ORAC values also demonstrate an ability to reduce radical formation and activity in cells.
Q: Does a high ORAC value guarantee a good CAA result?
A: No. There are a number of factors that might prevent a high-ORAC substance from performing well in the CAA test. For example, the substance might not be absorbed by the cell, or it might be toxic to the cell, which it is why it is valuable to test and find out.
Q: Does a low ORAC value mean that our product will have a good CAA result?
A: No. There will not be a perfect correlation between ORAC and CAA results. There are a number of factors that might cause a substance with a low ORAC value to have a significant CAA result. This is another reason that it is valuable to perform the combo testing.
Q: Are there other benefits to conducting the ORAC/CAA combo?
A: Yes. The test combination can be used in product development for screening formulations. It can also be a valuable pre-clinical tool for clinical protocol design.
Q: What if I have more questions?
A: Simply contact Brunswick Labs at firstname.lastname@example.org, or call +1-508-281-6660.